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发布于:2021-10-10 17:00:48  访问:45 次 回复:0 篇
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Fourfold upregulation in DyW animals compared to age-matched WT (P < 0.0001 by
Skeletal Muscle 2014, 4:14 http://www.skeletalmusclejournal.com/content/4/1/Page 12 of54965-21-8 Figure 7 Western blot and quantitative RT-PCR show upregulated extracellular matrix remodeling pathways in DyW mice throughout postnatal development. (D) Matrix metalloproteinase 2 (MMP-2) transcription levels became elevated by postnatal week 2 and remained that way Hygromycin B chemical information get CS-1974 through week 4 (P < 0.0005 by two-way ANOVA (n = 5)). Expression of MMP-2 in WT muscles began high and decreased with age, becoming significantly lower by week 3 (P < 0.001 by two-way ANOVA (n PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/16382199 = 5)) (Figure 7D), whereas MMP-9 showed no fluctuation over time (Figure 7E). In DyW mice, MMP-2 expression was consistent at weeks 1 through 3 and increased significantly by week 4 (P < 0.0001 by two-way ANOVA (n = 5).Fourfold upregulation in DyW animals compared to age-matched WT (P < 0.0001 by two-way ANOVA (n = 5)). Postn was also upregulated in DyW animals throughout most of development (Figure 7C). WT mice had their highest levels of Postn expression at postnatal week 1, which significantly decreased by week 3 (P < 0.0001 by two-way ANOVA (n = 5)). Like Fn1 expression, expression of Postn in DyW muscles showed a decrease betweenMehuron et al. Skeletal Muscle 2014, 4:14 http://www.skeletalmusclejournal.com/content/4/1/Page 12 ofFigure 7 Western blot and quantitative RT-PCR show upregulated extracellular matrix remodeling pathways in DyW mice throughout postnatal development. (A) Western blot of total and phosphorylated Smad2/3 (pSmad2/3) shows an increase in transforming growth factor (TGF-) signaling as early as week 1. Additionally, we saw a decrease in Smad7 expression as early as week 2. (DyW is also referred to as KO, Knockout.) (B) Fibronectin 1 (Fn1) gene expression was found to be upregulated two- to fivefold in Lama2DyW (DyW) animals at all four time points compared to age-matched wild-type (WT) mice (P < 0.0001 by two-way analysis of variance (ANOVA) and n = 5 for all groups). (C) Additionally, periostin (Postn) transcript levels showed massive upregulation at weeks 2, 3 and 4 (P < 0.05 for week 1, P < 0.005 for week 3 and P < 0.0001 for weeks 2 and 4, all by two-way ANOVA (n = 5 for all groups)). (D) Matrix metalloproteinase 2 (MMP-2) transcription levels became elevated by postnatal week 2 and remained that way through week 4 (P < 0.0005 by two-way ANOVA (n = 5)). (E) In contrast, MMP-9 expression was significantly upregulated at weeks 1, 2 and 3 (P < 0.005 by two-way ANOVA (n = 5)), but was comparable to the WT by week 4. (F) Gene expression of tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) shows a several-fold upregulation starting as early as week 1 (P < 0.0005 by two-way ANOVA (n = 5)). Asterisks indicate statistical significance between age-matched DyW and WT animals, hash tags indicate significant changes within the DyW group and Deltas indicate significant changes within the WT group. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 These statistical representations also apply other symbols (, #).Mehuron et al.
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